MM170
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MM170
Cell Line Name
Cell Line Description
Metastatic Melanoma. Cell Bank Australia reference number: CBA1346
Discontinued Message
This cell line has been found to have low level contamination and testing has revealed the presence of a second cell line in the earliest stock.
General Info
Species
Human
Links
Cellosaurus: CVCL_2603
Release Conditions
Characteristics
Tissue of Origin
Disease
Culture Conditions
Cell Type
Subculture Routine
Optimal split ratio 1:4 (seeding density 0.8 x10⁴ cells/cm²).Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
Culture Medium
RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) +10% FBS
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
R H Whitehead and J. H.Little Tissue Culture Studies on Human Malignant Melanoma Pigment Cell 1: 382-389, 1975
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: MM170 (ECACC 10092315).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.