NCI-H929
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NCI-H929
Cell Line Name
Cell Line Description
This IgA-producing cell line has been established from a malignant effusion in a 62-year-old Caucasian woman with myeloma. They are positive for plasma cell antigen 1 (PCA-1), transferrin receptor and CD38 but negative for early B cell developmental markers. HLA DR and CALLA. NCI-H929 is negative for Epstein-Barr virus nuclear antigen. A rearrangement of the c-myc proto-oncogene has been observed as well as the expression of c-myc RNA and an activated ras allele.
General Info
Species
Human
Other Collection No.
Links
Cellosaurus: CVCL_1600
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
DNA profile (STR Profile)
Amelogenin: X
CSF1PO: 11
D5S818: 11,12
D7S820: 10,12
D13S317: 12
D16S539: 9,13
TH01: 9.3
TPOX: 8,11
vWA: 14,15
Karyotype
Applications
Chemotherapeutic agent studies, receptor studies, carcinogenesis assays
Disease
Culture Conditions
Cell Type
Subculture Routine
Maintain cultures between 2-4 x 100,000 cells/ml; 5% CO₂; 37°C.
Culture Medium
RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Blood 1986;67:1542; ibid 1988;72:1163; Mol Cell Biol 1988;8:124
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: NCI-H929 (ECACC 95050415).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.