ECACC publications in collaboration with Durham University

In an ongoing collaboration between Durham University and the European Collection of Authenticated Cell Cultures (ECACC), two papers have been published utilising ECACC cells as model cell lines for 2D culture. 

In 2023, in a study on the impact of the physical cellular microenvironment on the structure and function of a model hepatocyte cell line for drug toxicity applications, HepG2 (ECACC 85011430) was used as a model cell line for HepG2 2D culture¹. The study examines the cellular processes that occur when a cell is cultured on 2D or 3D surfaces for a short period of time prior to its use in functional assays and evaluates the impact of the process of ‘mechanotransduction’ on alterations in gene expression, protein expression and functional capabilities.  

Most recently, in October 2024, a study was published on bioengineering the human intestinal mucosa which focussed on the importance of stromal support for pharmacological evaluation in vitro². This study used Caco-2 (ECACC 86010202 ) a human colorectal adenocarcinoma cell line which is commonly used in intestinal barrier models due to its ability to spontaneously differentiate adopting qualities representative of enterocytes. The study explores the link between stromal–epithelial crosstalk, paracrine communication, and the role of the keratinocyte growth factor (KGF) as a soluble mediator, underpinning the tissue-specific role of fibroblast subpopulations.  

Our Latest Publications

To keep up to date with publications from across Culture Collections, visit our publications page. 

References

1. Allcock, B., Wei, W., Goncalves, K., Hoyle, H., Robert, A., Quelch-Cliffe, R., Hayward, A., Cooper, J., & Przyborski, S. (2023). Impact of the Physical Cellular Microenvironment on the Structure and Function of a Model Hepatocyte Cell Line for Drug Toxicity Applications. Cells, 12(19), 2408. https://doi.org/10.3390/cells12192408  
2. Freer, M., Cooper, J., Goncalves, K., & Przyborski, S. (2024). Bioengineering the Human Intestinal Mucosa and the Importance of Stromal Support for Pharmacological Evaluation In Vitro. Cells, 13(22), 1859. https://doi.org/10.3390/cells13221859