Unique cell lines: GP2d and GP5d
ECACC has a number of unique human cell lines not available from any other culture collection. This collection contains cell lines representing several different diseases, hybridomas, HLA-typed panels and a series of transformed lymphoblastoid cell lines representing human random controls.
Two examples of our unique lines are human colon carcinoma lines derived from the same donor:
Cell line information:
Derived from a human colon adenocarcinoma, GP2d and GP5d have been established from the same adenocarcinoma. The cells were derived from a local recurrence of Duke's grade B, poorly differentiated carcinoma of the colon from a 71 year old female at surgical resection.
The patient received no preoperative chemotherapy or radiotherapy and had a strong family history of colon cancer with two first degree relatives dying of the disease below the age of 55 years.
Both clones possess genetic changes consistent with the pattern of tumour progression in colon cancer but are morphologically distinct. An interstitial deletion on chromosome 5 (region 14q to 22q) and an inverted duplication of bands 10q11 and 10q21 has been reported. Both cell lines contain a normal copy of the ki-ras gene and a copy with a transition in codon 12. GP2d cells show growth response to EGF, TGF alpha or insulin with an increase in cell numbers. Amphiregulin mRNA is abundant in GP2d but almost undetectable in GP5d.
STR profile:
Amelogenin: X
CSF1PO: 9,12
D13S317: 8,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 7,9.3
TPOX: 8,11
vWA: 17,18
Subculture routine:
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 5x10,000 to 1x100,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
References:
Additional Bibliography:
Written by Edward Burnett