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UK Health Security Agency

1-5c-4 (HeLa deriative)

1-5c-4 (HeLa deriative)

Catalogue No.

88021103

Cell Line Name

1-5c-4 (HeLa deriative)

Cell Line Description

These cells were originally Chang conjunctival cells adapted by Wong and Kilbourne, and designated as conjunctival 'D' cells. Susceptible to swine influenza viruses, supports production of plaque formation by a variety of influenza virus subtypes. Has HeLa markers, exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Epithelial with occasional giant cells. Ethnicity: Black.

Characteristics

Tissue of Origin

Cervix

Karyotype

2n = 46

Applications

Plaque assays, cloning, genetic and virus studies: swine influenza viruses

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using EDTA or 0.05% trypsin; 5% CO₂; 37°C.

Culture Medium

Medium 199 (HBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Exp Med 1961;95:113; In Vitro 1978;14:469; Proc Soc Exp Biol Med 1954;87:440; Nature 1976;259:211

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 1-5c-4 (HeLa deriative) (ECACC 88021103).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.