1.1E7
1.1E7
Cell Line Name
Cell Line Description
1.1E7 is a hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line (ECACC catalogue number 87092802). 1.1E7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology. 1.1E7 cells provide a method of producing pure insulin secreting cells when stimulated (please see attached protocols for the stimulation of insulin secretion).. This is an alternative to the use of primary tissue in cell transplantation therapies for type 1 diabetes. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Discontinued Message
Interspecies testing confirmed the presence of human and rat nuclear DNA sequences in 1.1E7
General Info
Species
Human
Links
Cellosaurus: CVCL_2257
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
DNA profile (STR Profile)
STR-PCR Data: Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 8 THO1: 7,8 TPOX: 8,11 vWA: 15
Karyotype
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 23hrs. At confluence 10⁵ cells/cm² can be expected.
Culture Medium
RPMI-1640 + 2mM Glutamine + 10% FCS
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Lieber M et al., (1975) Establishment of a continuous tumor cell line (panc-1) from a carcinoma of the exocrine pancreas. Int J Cancer 15: 741-747 PMID: 1140870
Bibliography
McCluskey JT, Hamid M, Parke HG, McClenaghan NH, Gomis R, Flatt PR. 2011 Development and functional characterisation of insulin-releasing human pancreatic beta cell lines produced by electrofusion. J Biol Chem. Apr 22. [Epub ahead of print] PMID: 21515691. Guo-Parke H, McCluskey JT, Kelly C, Hamid M, McClenaghan NH, Flatt PR.2012. Configuration of electrofusion-derived human insulin-secreting cell line as pseudoislets enhances functionality and therapeutic utility. J Endocrinol. 214(3):257-65. PMID: 22685334
Documents
- 10070101_1.1E7_Growth Profile
- Human pancreatic beta cell lines culture and insulin assay protocols-updated July-2014
- Passage numbers explained
- MSDS for nucleic acids
- MSDS For Frozen Cell Culture
- M061 University of Ulster Deposit Release Form for Commercial Organisations
- Important technical information for successful culture of your cells
Images
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: 1.1E7 (ECACC 10070101).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.