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1301

1301

Catalogue No.

01051619

Cell Line Name

1301

Cell Line Description

1301 is a sub-line of CCRF-CEM, ECACC catalogue no. 85112105. It has unusually long telomeres, useful in tests where telomere length is determined, where it can be used as control cells with known telomere length. This cell line has been recommended as a control cell line for use with the Telomere PNA Kit/FITC for flow cytometry from DakoCytomation.

Characteristics

Tissue of Origin

Blood

Karyotype

Tetraploid

Applications

Internal control cell in telomere measurements performed by flow cytometry.

Disease

Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 37°C; 5% CO₂.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr. T. Just, Dako A/S,Dako A/SProduktionsvej 42DK2600Glostrup Denmark

Country of Origin

Denmark

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Larsson, I. et al: Develop. Biol. Standard 1979;42:193-197.

Bibliography

Hultdin M, Grönlund E, Norrback K, Eriksson-Lindström E, Just T, Roos G 1998 Telomere analysis by fluorescence in situ hybridization and flow cytometry. Nucleic Acids Res.15;26(16):3651-6 PMID: 9685479

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 1301 (ECACC 01051619).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.