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143B

143B

Catalogue No.

91112502

Cell Line Name

143B

Cell Line Description

Cells are thymidine kinase deficient (TK-) and resistant to BUdR.143B and HOS (ECACC Catalogue number 87070202) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+(Skovgaard et al 2010 PMID: 20544518).

Characteristics

Tissue of Origin

Bone

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D5S818: 13
D7S820: 11,12
D13S317: 12
D16S539: 10,13
TH01: 6
TPOX: 11
vWA: 18

Karyotype

Not specified

Applications

Tumourigenicity studies

Disease

Osteosarcoma

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 15µg/ml Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None specified by depositor

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 143B (ECACC 91112502).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.