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UK Health Security Agency

1G2F8

1G2F8

Catalogue No.

21043001

Cell Line Name

1G2F8

Cell Line Description

1G2F8 is a mouse x mouse hybridoma cell line which produces an antibody that recognises the bacterial Streptococcus pneumonia antigen DnaK (Hsp70).

Characteristics

Myeloma

Sp2/0-Ag14

Immunogen

Peptide from bacterial DnaK

Immunological Donor

Mouse

Antibody Isotype

Isotype was confirmed as IgG1 by ECACC

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

Resuscitate cells at the higher end of the seeding density (2-9 x 105 cells/ml). When seeding into small volumes, the cell culture flask can be position upright in the incubator. Return to a horizontal position once culture is established.

Upon dilution of cells, seed @ 2-3 x 105 cells/ml and routinely passage twice weekly. Cells can be grown in Corning cell culture flasks and cell factories, however cells can be semi adherent and viability can be reduced when re-suspending cells.

Maintain sub-confluent cultures to retain viability of culture (<9 x 105 cells/ml).

Harvest and passage: remove media carefully (leaving about 0.5-1.0mL) without disturbing cells. Check layer of cells and re-suspend by tapping flask, re-suspend and count.

To prepare antibody: use of cell factories with twice weekly harvest once culture is established.

Cells also grow well in Ultra Low Attachment plastic ware (ULA flasks). Here cells can grow in single suspension and as small cell aggregates.

Growth conditions: 37°C, 5% CO2.

Culture Medium

RPMI + 2mM L-Glutamine + 10% Foetal Bovine Serum (FBS)

Resuscitation: Ensure 20% FBS is used as recover can be very slow. Reduce to 10% once a growing culture is established.

To freeze: 10% DMSO + 90% FBS.

Growth Mode

Semi-adherent

Additional Info

Depositor

Claire Entwisle, ImmunoBiology Ltd,

Country of Origin

United Kingdom

Applications

Detection of PspA Protein by ELISA.

GMO Status

Not Applicable

Hazard Group (ACDP)

2

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 1G2F8 (ECACC 21043001)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.