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UK Health Security Agency

293T

293T

Catalogue No.

12022001

Cell Line Name

293T

Cell Line Description

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

Characteristics

Products

SV40 T antigen

Tissue of Origin

Kidney (embryo)

Morphology

Small slightly rounded cells growing in a dispersed manner

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 8,9
D7S820: 11
D13S317: 12
D16S539: 9,13
TH01: 7,9.3
TPOX: 11
vWA: 16,19

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x104 cells/cm² using 0.05% trypsin/EDTA.

 

Recommended growth conditions 5% CO2; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Professor Michele Calos, Department of Genetics, Alway Building, Room M-334 Stanford University School of Medicine 300 Pasteur Drive Stanford, CA 94305-5120.

Country of Origin

United States

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

DuBridge RB, Tang P, Hsia HC, Leong PM, Miller JH, Calos MP. 1987 Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol Cell Biol. 7(1):379-87.PMID: 3031469.

Bibliography

Pear WS, Nolan GP, Scott ML, Baltimore D. 1993 Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci U S A. 90(18):8392-6. PMID: 7690960. Sena-Esteves M, Saeki Y, Camp SM, Chiocca EA, Breakefield XO. 1999 Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J Virol. 73(12):10426-39.PMID: 10559361.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 293T (ECACC 12022001).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.