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UK Health Security Agency

3T3-F442A

3T3-F442A

Catalogue No.

00070654

Cell Line Name

3T3-F442A

Cell Line Description

Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 hours. We would like to manage customer expectations with regard to the potential of the current 3T3 cell line stocks to differentiate into adipocytes. If you intend to use the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g. 2 - 5 weeks, and the proportion of the population which differentiates can be limited. If you have previously used 3T3 cells from an alternative source we cannot guarantee the differentiation performance will be the same.

Characteristics

Tissue of Origin

Adipose

Karyotype

Not specified

Applications

Once terminally differentiated the cells can be used as a model for either adipocyte differentiation or mature adipocytes.

Disease

None Stated

Culture Conditions

Cell Type

Fibroblast-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3-1:5 ie. seeding at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA, 5% CO₂, 37°C. If cells are allowed to become confluent they will differentiate into adipocytes. If cryopreserving these cells, use Newborn calf serum NCS + 10% DMSO, freeze down at 30-50% confluency.

Culture Medium

DMEM + 10% Newborn Calf Serum (NCS) + 2mM Glutamine

Growth Mode

Adherent

Additional Info

Depositor

Dr Karen Chapman, University of Edinburgh, Department of Molecular Endocrinology, Western General Hospital, Edinburgh EH4 2XU

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Green and Kehinde 1974. Cell 113-116, Green and Kehinde (1975) Cell 5 19-27, Green and Kehinde (1976) Cell 7 105-113.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 3T3-F442A (ECACC 00070654).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.