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UK Health Security Agency

833KE

833KE

Catalogue No.

06072611

Cell Line Name

833KE

Cell Line Description

A human embryonal carcinoma cell line derived from a human testicular germ cell tumour.

Characteristics

Tissue of Origin

Embryonal carcinoma

Karyotype

Not specified

Disease

Carcinoma

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%), approximately every 3-4 days, 1:3 to 1:5 i.e. seeding at >6x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 10% CO₂; 37°C. Cells need to be maintained at a high cell density.

Culture Medium

DMEM (High Glucose) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). No sodium pyruvate.

Growth Mode

Adherent

Additional Info

Depositor

Professor Peter Andrews, Department of Biomedical Sciences, Centre for Stem Cell Biology, The University of Sheffield, UK.

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Hiraoka et al., (1988) Ultrastructure of four human germ cell tumor-derived cell lines: effect of 12-O-tetradecanoyl phorbol-13-acetate. Cancer Invest 6:393-402.

Bibliography

Mavilio et al., (1988) Activation of four homeobox gene clusters in human embryonal carcinoma cells induced to differentiate by retinoic acid. Differentiation 37:73-9. Andrews et al., (1994) Inhibition of proliferation and induction of differentiation of pluripotent human embryonal carcinoma cells by osteogenic protein-1 (or bone morphogenetic protein-7). Lab Invest 71:243-51. Spierings et al., (2003) Loss of drug-induced activation of the CD95 apoptotic pathway in a cisplatin-resistant testicular germ cell tumor cell line. Cell Death Differ 10:808-22.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 833KE (ECACC 06072611).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.