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UK Health Security Agency

92-1

92-1

Catalogue No.

13012458

Cell Line Name

92-1

Cell Line Description

92-1 (ESTDAB-127) was established from a primary uveal melanoma in the right orbit of a 76 year old female. The 92-1 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission's fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB (http://www.ebi.ac.uk/ipd/estdab) contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. This information is appended below. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation. Robinson J, Waller MJ, Fail SC, Marsh SG. 2006 The IMGT/HLA and IPD databases. Hum Mutat 27(12):11929.PMID: 16944494. Pawelec G, Marsh SG. 2006 ESTDAB: a collection of immunologically characterised melanoma cell lines and searchable databank. Cancer. Immunol Immunother. 55(6):623-7.PMID: 16421722. Robinson J, Roberts CH, Dodi IA, Madrigal JA, Pawelec G, Wedel L, Marsh SG.2009 The European searchable tumour line database. Cancer. Immunol Immunother. 58(9):1501-6. PMID: 19172270.

Characteristics

Receptors

see http://www.ebi.ac.uk/ipd/estdab

Tissue of Origin

Eye (uveal melanoma)

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10,11
D5S818: 9,11
D7S820: 10,11
D13S317: 11,12
D16S539: 12
TH01: 9,9.3
TPOX: 8,9
vWA: 16

Karyotype

see http://www.ebi.ac.uk/ipd/estdab

Disease

Melanoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 1-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 10 x10,000 cells/cm²

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Martine Jager, Department of Opthalmology, Leiden University Medical Centre, PO Box 9600, 2300 RC Leiden, The Netherlands.

Country of Origin

Netherlands

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

De Waard-Siebinga I, Blom DJ, Griffioen M, Schrier PI, Hoogendoorn E, Beverstock G, Danen EH, Jager MJ. 1995 Establishment and characterization of an uveal-melanoma cell line. Int J Cancer. 62(2):155-61.PMID: 7622289.

Bibliography

de Waard-Siebinga I, Creyghton WM, Kool J, Jager MJ 1995 Effects of interferon alfa and gamma on human uveal melanoma cells in vitro. Br J Ophthalmol. 79(9):847-55. PMID: 7488605. Ma D, Luyten GP, Luider TM, Jager MJ, Niederkorn JY. 1996 Association between NM23-H1 gene expression and metastasis of human uveal melanoma in an animal model. Invest Ophthalmol Vis Sci. 37(11):2293-301. PMID: 8843913 Ren DH, Mayhew E, Hay C, Li H, Alizadeh H, Niederkorn JY 2004 Uveal melanoma expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors and susceptibility to TRAIL-induced apoptosis. Invest Ophthalmol Vis Sci. 45(4):1162-8. PMID: 15037583. Abdel-Rahman MH, Boru G, Massengill J, Salem MM, Davidorf FH 2010 MET oncogene inhibition as a potential target of therapy for uveal melanomas. Invest Ophthalmol Vis Sci. 2010 Jul;51(7):3333-9.PMID: 20164465.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 92-1 (ECACC 13012458).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.