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UK Health Security Agency

A10-85

A10-85

Catalogue No.

93040117

Cell Line Name

A10-85

Cell Line Description

The glioma cell line A10-85 is a subclone of A10 cells which originally emerged from the parent line IA2 by reducing the serum concentration from 15% to 10%. This parent line IA2 was derived from a rat brain glioma induced transplacentally by ethylnitroso urea (inbred BDIxrats). A10-85 cells form processes in response to cAMP. GAPDH can be induced by hydrocortisone succinate and good growth was obtained in semi-solid medium. Injection of cells into syngeneric animals resulted in the formation of malignant astrocytic tumours after short latent periods.

Characteristics

Tissue of Origin

Glioma

Karyotype

Modal no. 42

Applications

Experimental tumour models, carcinogenesis studies

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:50 to 1:100 seeding at 5x10,000 to 1x100,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr J Roscoe, Department of Histopathology, University College and Middlesex School of Medicine, London

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Br J Exp Path 1979;60:209

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: A10-85 (ECACC 93040117).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.