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UK Health Security Agency

AB-1 HA

AB-1 HA

Catalogue No.

12070506

Cell Line Name

AB-1 HA

Cell Line Description

AB-1 HA is a mouse mesothelioma cell line transfected with influenza haemagglutinin gene (HA). Mice (BALB/c strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. The resulting celll ine, designated AB-1 was transfected with influenza haemagglutinin gene (HA) in order to generate an endogenous tumour antigen. This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by Cell Bank Australia. Cell Bank Australia reference number: CBA-1374

Characteristics

Tissue of Origin

Mesothelium

Morphology

Small and can be spindly at low density but form a uniform cobblestone epithelial layer at near confluence

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Optimal split ratio 1:10 - 1:20 (seeding density 0.7 x10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. Cells will require passaging twice weekly at this seeding density.

Culture Medium

RPMI1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 10% FCS + 400 µg/ml G418

Growth Mode

Adherent

Additional Info

Depositor

Richard Lake - University of Western Australia, Australia

Country of Origin

Australia

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Marzo AL, Lake RA, Robinson BW, Scott B. 1999 T-cell receptor transgenic analysis of tumor-specific CD8 and CD4 responses in the eradication of solid tumors. Cancer Res. 59(5):1071-9 PMID: 10070965.

Bibliography

Davis MR, Manning LS, Whitaker D, Garlepp MJ, Robinson BW. 1992 Establishment of a murine model of malignant mesothelioma. Int J Cancer. 52(6):881-6 PMID: 1459729.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: AB-1 HA (ECACC 12070506).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.