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UK Health Security Agency

ABSa15

ABSa15

Catalogue No.

13112201

Cell Line Name

ABSa15

Cell Line Description

The ABSa15 cell line is derived from the calcified branchial arches of a gilthead seabream and is capable of extracellular matrix mineralization. Gene expression analysis is suggestive of pre-chondrocyte lineage. Extracellular matrix mineralization occurs on supplementation with calcium chloride, β-glycerophosphate and ascorbic acid. Mineral deposits can be detected as early as two weeks of treatment and increase for up to four weeks. ABSa15 is useful for investigating the mechanism of cell differentiation, extracellular matrix mineralization and also the screening of mineralogenic molecules.

Characteristics

Tissue of Origin

Branchial arches (calcified cartilage)

Morphology

Polygonal at confluence

Karyotype

48

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like (with a polygonal morphology at confluence)

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 1-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 10% CO₂; 33°C. Saturation density approx 8x10,000 cells/cm² .

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

M. Leonor Cancela & Vincent Laizé, Centre of Marine Sciences (CCMAR) & Department of Biomedical Sciences & Medicine (DCBM), University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.

Country of Origin

Portugal

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Marques CL, Rafael MS, Cancela ML, Laizé V. 2007 Establishment of primary cell cultures from fish calcified tissues. Cytotechnology. 55(1):9-13. PMID: 19002990.

Bibliography

Tiago DM, Marques CL, Roberto VP, Cancela ML, Laizé V 2014 Mir-20a regulates in vitro mineralization and BMP signalling pathway by targeting BMP-2 transcript in fish. Arch Biochem Biophys. 543: 23-30. doi: 10.1016/j.abb.2013.12.009. Epub 2013 Dec 21. PMID: 24361749.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: ABSa15 (ECACC 13112201).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.