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UK Health Security Agency

AC29

AC29

Catalogue No.

21102109

Cell Line Name

AC29

Cell Line Description

AC29 is a mouse mesothelioma cell line.


Mice (CBA strain, female, 6-8 weeks old) were exposed to crocidolite asbestos through intraperitoneal injection, resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid.

This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by Cell Bank Australia. Cell Bank Australia reference number: CBA-1664

Characteristics

Tissue of Origin

Ascites fluid

Morphology

Epithelial-like

Disease

Mesothelioma

Culture Conditions

Cell Type

Mesothelium

Subculture Routine

Remove protective cryoflex layer around the ampoule prior to thawing.


Thaw the ampoule by gently agitating in a 37oC waterbath; thawing should be rapid (around 2 minutes).


A centrifugation step to remove the cryoprotectant after thawing is necessary for this cell line.


For Lot 16640911E thaw 1 vial into 1xT75


Subculturing Procedure:


Medium Renewal: 2-3 times per week.


Subcultivation ratio: 1:8-1:16, Seeding density 0.6-1.0 x104 cells/cm2


Split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes.


Culture conditions: Incubate the culture at 37oC with 5% CO2.


Cryoprotectant Medium: 10% DMSO + 90% FCS

Culture Medium

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES)
+ 5% Foetal Calf Serum

Growth Mode

Adherent

Additional Info

Depositor

Richard Lake - University of Western Australia

Country of Origin

Australia

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Original Reference
Davis MR, Manning LS, Whitaker D, Garlepp MJ, Robinson BW (1992)
Establishment of a murine model of malignant mesothelioma.
Int J Cancer 52: 881-886.
PubMed: 1459729

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: AC29 (ECACC 21102109).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.