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UK Health Security Agency

AR42J

AR42J

Catalogue No.

93100618

Cell Line Name

AR42J

Cell Line Description

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

Characteristics

Receptors

Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin

Products

Amylase and exocrine enzymes

Tissue of Origin

Pancreas

Morphology

Clumps of cells

Karyotype

Not specified

Applications

Enzyme and receptor studies

Disease

Neoplasms

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Cells grow in hollow spheroid colonies that can attach loosely. Maintain cultures between 5000-20000 cells/cm²; 5% CO₂; 37°C. Cell viability may be poor on resuscitation from frozen (approx 50%) and growth can be slow. Cells may take up to 7 days to achieve 70% confluence. Resuscitate using 20% FBS, seeding at 10000 cells/cm² and media change after 48 hours. Adherent cells should be removed using 0.05% Trypsin/EDTA. N.B. High cell viability cannot be expected during culture.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Semi-adherent aggregates

Additional Info

Depositor

Dr S Watson, Department of Surgery, Queens Medical Center, Nottingham

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

In Vitro 1980;16:212

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: AR42J (ECACC 93100618).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.