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UK Health Security Agency

B95a

B95a

Catalogue No.

01092505

Cell Line Name

B95a

Cell Line Description

B95a is a derivative of the cell line B95-8 and was adapted to growth in adherence to the substrate surface. The parent line B95-8 was derived from a cotton-top marmoset (Saguinus oedipus). The B-lymphoblastoid cells are transformed with Epstein-Barr virus (EBV). B95a has been reported to be 10,000-fold more sensitive to measles virus present in clinical specimens than Vero cells and are useful host cells for the isolation of measles virus.

General Info

Species

Monkey

Release Conditions

Restricted - CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the United Kingdom

Characteristics

Tissue of Origin

Blood

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 every 3-4 days using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C; cells grow initially as a monolayer, but will pile up and detach in maintenance medium.

Culture Medium

DMEM + 2mM Glutamine + 5%Foetal Bovine Serum (FBS) (for growth medium) or 2% FBS (for maintenance medium)

Growth Mode

Semi-adherent

Additional Info

Depositor

Dr. B.J. Cohen, Enteric and Respiratory Virus Laboratory, Central Public Health Laboratory, London

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Virol 1990: 64;700

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: B95a (ECACC 01092505).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.