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UK Health Security Agency

BGM

BGM

Catalogue No.

90092601

Cell Line Name

BGM

Cell Line Description

Used for the isolation of water borne viruses e.g. polio 1, 2, 3, Echo 3, 6, 7, 9, 12, 27, Coxsackie A9 + B1, 2, 3 and Reo 1.

General Info

Species

Monkey

Release Conditions

Restricted - CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the United Kingdom

Characteristics

Tissue of Origin

Kidney

Karyotype

Not specified

Applications

Virus studies: polio, Echo, Coxsackie and Reo viruses

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr S Clark, CAMR, Porton Down, Salisbury

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Health Lab Science 1974;11:275

Bibliography

(1)J Med Microbiol; 1995 Dec: 43(6): 411-21"Ultrastructural variation of cultured Ehrlichia chaffeensis."(2)Microbiol Res; 1995 Sep: 150(3): 247-63"The impact of interfacial interactions on poliovirus infectivity in detergent-treated cell monolayer cultures."(3)Folia Biol (Praha); 1994: 40(3): 125-33"Rapid distinguishing of three African green monkey cell lines: different morphology of chromosomes with nucleolus organizer regions (NORs)."(4)Zentralbl Hyg Umweltmed; 1994 Aug: 196(1): 38-51"Occurrence and isolation of human enteroviruses from the air of waste removal and disposal plants"(5)Vet Microbiol; 1993 Oct: 37(1-2): 65-83"Comparison of type I and type II Chlamydia psittaci strains infecting koalas (Phascolarctos cinereus)."(6) Isr J Med Sci; 1993 Jan: 29(1): 2-6"Inhibitory effect of the calcium antagonist, verapamil, on measles and vaccinia replication in cell culture."(7)Acta Virol; 1992 Jan: 36(1): 13-8"The effect of monocyte-derived macrophages on the growth of Rickettsia conorii in permissive cells."(8)Med Lab Sci; 1992 Mar: 49(1): 60-5"Isolation of enteroviruses and adenoviruses in continuous simian cell lines."(9)J Virol Methods; 1992 Oct: 40(1): 67-75"Processing procedures for recovering enteric viruses from wastewater sludges."(10)Avian Dis; 1992 Apr-Jun: 36(2): 415-22"Effect of cell-culture passage on the pathogenicity and immunogenicity of two variant strains of infectious bursal disease virus."(11) Arch Inst Pasteur Tunis; 1992 Jan-Apr: 69(1-2): 13-9"Cytotoxicity of ferric chloride used as an adjuvant of organic acid precipitation"(12)Berl Munch Tierarztl Wochenschr; 1992 Feb 1: 105(2): 45-9"New possibilities for the diagnosis of Q fever and for the differentiation of the causative agent"(13)Biologicals; 1991 Jul: 19(3): 213-8"Possibilities of vaccine manufacture in human diploid cell strains with a serum replacement factor."(14) Avian Dis; 1991 Jan-Mar: 35(1): 3-7"Isolation of infectious bursal disease virus from turkeys with arthritic and respiratory symptoms in commercial farms in Quebec."(15)Tierarztl Prax; 1991 Oct: 19(5): 475-9"Bacteriologic studies of aborted sheep fetuses with special reference to chlamydiae"(16)Bull Acad Natl Med; 1991 Mar: 175(3): 439-52"Plants as a source of research in antiviral activity. Example of the Haemanthus albiflos bulb"(17) Avian Dis; 1990 Jan-Mar: 34(1): 141-5"Infectious bursal disease virus variant from commercial Leghorn pullets."(18)Appl Environ Microbiol; 1990 Jan: 56(1): 295-7"Enhancement of enterovirus infectivity in vitro by pretreating host cell monolayers with the cationic polymer polyethyleneimine."(19)J Gen Microbiol; 1989 Jun: 135 ( Pt 6): 1619-23"Inhibition of growth of Chlamydia trachomatis by the calcium antagonist verapamil."(20)J Clin Microbiol; 1989 Oct: 27(10): 2364-5"Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system."(21)J Clin Microbiol; 1988 Dec: 26(12): 2576-80"Comparison of cell cultures for rapid isolation of enteroviruses."(22)Vet Microbiol; 1988 Sep: 18(1): 15-25"Comparison of DNA-spot hybridization, cell culture and direct immunofluorescence staining for the diagnosis of avian chlamydiae."(23) Can J Microbiol; 1987 Jun: 33(6): 568-70"Detection and quantitation of human enteric viruses in waste waters: increased sensitivity using a human immune serum globulin--immunoperoxidase assay on MA-104 cells."(24)Avian Dis; 1987 Apr-Jun: 31(2): 370-5"Replication of infectious bursal disease virus in continuous cell lines."(25) Zentralbl Bakteriol Mikrobiol Hyg [A; 1987 Nov: 267(1): 67-73"Detection of Coxiella burnetii by the immunoperoxidase technique."(26) Can J Microbiol; 1986 Dec: 32(12): 922-5"Elimination of human enteric viruses during conventional waste water treatment by activated sludge."(27)J Med Virol; 1986 Jul: 19(3): 219-28"A combination of four cell types for rapid detection of enteroviruses in clinical specimens."(28) Appl Environ Microbiol; 1986 May: 51(5): 1036-40"Evaluation of mixed cell types and 5-iodo-2'-deoxyuridine treatment upon plaque assay titers of human enteric viruses."(29)Appl Environ Microbiol; 1986 Apr: 51(4): 790-812"Optimization of the BGM cell line culture and viral assay procedures for monitoring viruses in the environment."(30) Lipids; 1986 Sep: 21(9): 608-11"Imprints of virus infection: can paramyxoviruses permanently modify triacylglycerol metabolism?"(31) Can J Microbiol; 1985 Nov: 31(11): 977-80"Influence of inoculum size, incubation temperature, and cell culture density on virus detection in environmental samples."(32)Appl Environ Microbiol; 1985 Nov: 50(5): 1308-10"Immunoperoxidase method with human immune serum globulin for broad-spectrum detection of cultivable human enteric viruses: application to enumeration of cultivable viruses in environmental samples."(33)Appl Environ Microbiol; 1985 Aug: 50(2): 280-6"Enteroviruses in sludge: multiyear experience with four wastewater treatment plants."(34) Diagn Microbiol Infect Dis; 1984 Sep: 2(4): 353-7"The isolation of nonpolio enteroviruses from serum."(35)Appl Environ Microbiol; 1984 Feb: 47(2): 378-80"Trypsin-treated Ma-104: a sensitive cell line for isolating enteric viruses from environmental samples."(36) Can J Microbiol; 1983 Dec: 29(12): 1731-5"Attachment of Salmonella to mammalian cells in vitro."(37)Biochem J; 1983 Sep 15: 214(3): 665-70"Measles-virus-persistent infection in BGM cells. Modification of the incorporation of 3Harachidonic acid and 14C]stearic acid into lipids."(38)Appl Environ Microbiol; 1982 Jun: 43(6): 1425-33"Microcystis aeruginosa toxin: cell culture toxicity, hemolysis, and mutagenicity assays."(39) Appl Environ Microbiol; 1982 Apr: 43(4): 740-6"Evaluation of cell lines and immunofluorescence and plaque assay procedures for quantifying reoviruses in sewage."(40)J Gen Virol; 1982 Nov: 63 (Pt 1): 247-50"Synthesis of a unique polypeptide in measles virus-infected BGM cells."(41)J Gen Virol; 1982 Oct: 62 (Pt 2): 249-58"Phospholipids in a measles virus persistent infection: modification of fatty acid metabolism and fatty acid composition of released virus."(42) Appl Environ Microbiol; 1982 Jun: 43(6): 1413-8"Comparison of methods for recovering indigenous viruses from raw wastewater sludge."(43) Arch Virol; 1981: 67(4): 297-308"Measles virus: evolution of a persistent infection in BGM cells."(44)J Virol; 1979 Sep: 31(3): 810-5"Simian rotavirus SA11 replication in cell cultures."(45)J Gen Virol; 1979 Oct: 45(1): 185-94"Changes in the virus-host cell relationship in a stable non-virogenic cell line persistently infected with measles virus (BGM/MV)."(46)Can J Microbiol; 1979 Mar: 25(3): 335-9"Response of various cell lines to Escherichia coli toxic products."(47)Intervirology; 1979: 11(5): 275-81"A study of the measles virus-induced proteins incorporated into the cell membrane."(48)Cell Differ; 1978 Dec: 7(6): 367-74"Sulfated glycosaminoglycans of cells grown in culture: dermatan sulfate disappearance in successive fibroblast subcultures."(49)J Gen Virol; 1978 Apr: 39(1): 113-24"Establishment and characterization of a subacute sclerosing panencephalitis (measles) virus persistent infection in BGM cells."(50)Health Lab Sci; 1976 Apr: 13(2): 115-7"Comparative sensitivity of the BGM cell line for isolation of enteric viruses."

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: BGM (ECACC 90092601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.