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UK Health Security Agency

BPAEC

BPAEC

Catalogue No.

86123101

Cell Line Name

BPAEC

Cell Line Description

BPAEC is susceptible to bovine respiratory viruses and is positive for BVDV.

Characteristics

Tissue of Origin

Lung

Karyotype

Not specified

Applications

Virus studies: bovine respiratory viruses

Disease

None Stated

Culture Conditions

Cell Type

Endothelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM or 199 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr A Hussein, Dept. of Paediatrics, John Radcliffe Hospital, Oxford

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None specified by depositor

Bibliography

(1)J Infect Dis; 1995 Oct: 172(4): 1119-22 "Escherichia coli endotoxin-mediated endothelial injury is modulated by glutathione ethyl ester." (2) Am J Respir Cell Mol Biol; 1995 Oct: 13(4): 442-8 "Nitric oxide increases cellular glutathione levels in rat lung fibroblasts." (3) J Cardiovasc Pharmacol; 1993: 22 Suppl 8: S38-41 "Regulation of endothelin-1 expression in normal and transfected endothelial cells." (4) Biochem Biophys Res Commun; 1993 Dec 30: 197(3): 1465-74 " Effects of transforming growth factor beta and interleukin-1 beta on expression of cyclooxygenase 1 and 2 and phospholipase A2 mRNA in lung fibroblasts and endothelial cells in culture." (5) Biochem Biophys Res Commun; 1993 Feb 26: 191(1): 134-41 "Phosphatidic cid directly activates endothelial cell protein kinase C." (6) J Cell Physiol; 1992 Oct: 153(1): 62-75 " Protein kinase C phosphorylates caldesmon77 and vimentin and enhances albumin permeability across cultured bovine pulmonary artery endothelial cell monolayers." (7) Am J Physiol; 1992 Jul: 263(1 Pt 1): L27-36 "Restricted diffusion of macromolecules by endothelial monolayers and small-pore filters." (8)J Cell Physiol; 1991 Dec: 149(3): 575-84 "Lectin binding to gp60 decreases specific albumin binding and transport in pulmonary artery endothelial monolayers published erratum appears in J Cell Physiol 1992 Jun;151(3):642" (9) Am J Respir Cell Mol Biol; 1991 May: 4(5): 408-16 "Relationship of oxidant-mediated cytotoxicity to phospholipid metabolism in endothelial cells."

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: BPAEC (ECACC 86123101).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.