skip to main content
UK Health Security Agency

C17.2

C17.2

Catalogue No.

07062902

Cell Line Name

C17.2

Cell Line Description

An immortalised mouse neural progenitor cell line capable of differentiation in vitro. The cell line was established by retorviral-mediated transduction of the avian myc oncogene into mitotic progenitor cells of neonatal mouse cerrebellum. Mouse strain CD1 x C57BL/6. This cell line is a valuable tool for in vitro and in vivo studies aimed at understanding the control of cell fate and differentiation of neural progenitors. The MMLV retrovirus vector used for the immortalisation process contained a neo resistance gene transcribed from an internal SV40 promoter. Therefore the cells are neo resistant. The morphology of the cells may change over time. Cells plated at low density may tend to become more process bearing whereas those plated more densely may tend to become flat and non-process bearing.

Characteristics

Tissue of Origin

Cerebellum

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Neuronal

Subculture Routine

Feed cells weekly with 50% conditioned medium and 50% fresh medium or split 1:10-1:20 in fresh medium using trypsin/EDTA. 5% CO₂; 37°C. Split sub-confluent cultures 1:10 - 1:20 i.e. seeding at 2-4 x 10,000 cells/cm². Flasks should be pre-coated with poly-L-lysine at 10 micrograms/ml in sterile distilled water. Cells can be split as dilute as 1:50 , but the phenotype may change i.e. cells may appear flat with an epithelial-like morphology which usually do not stain for neurofilament or GFAP.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Flasks should be pre-coated with poly-L-lysine at 10µg/ml in sterile distilled water.

Growth Mode

Adherent

Additional Info

Depositor

Constance L Cepko, Department of Genetics and Howard Hughes Medical Institute, Harvard Medical School, Boston MA 02115

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Ryder et al., (1990) Establishment and characterization of multipotent neural cell lines using retrovirus vector-mediated oncogene transfer. Journal of Neurobiology. 21, 2, 356-375

Bibliography

Snyder et al., (1992) Multipotent neural cell lines can engraft and participate in development of mouse cerebellum. Cell, Vol. 68, 33-51

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: C17.2 (ECACC 07062902).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.