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C2C12

C2C12

Catalogue No.

91031101

Cell Line Name

C2C12

Cell Line Description

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

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This cell line grows quickly and differentiates quickly. It needs to be seeded at a low and density kept sub confluent below 80% maximum.

Characteristics

Tissue of Origin

muscle

Karyotype

Not specified

Applications

Differentiation studies

Disease

None Stated

Culture Conditions

Cell Type

Myoblast

Subculture Routine

Cells can be relatively slow growing when resuscitated from frozen taking 4-5 days to reach 50% confluence when seeded at 2x1,000 cells/cm² . Split semi-confluent cultures (50 - 70%) 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence as myotube formation may occur.

Culture Medium

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Nature 1977;270:725, Science 1985;230;758-766, J. Cell Biol. 1994; 127:1755-1766[published erratum appears in J Cell Biol 1995 Feb;128(4):following 713], J.Virol. 1997;71:169-178, Proc. Natl. Acad.Sci. USA 1996;93:14082-14087, J. Biol. Chem. 1996;271:1386

Bibliography

(1)J Anim Sci; 1996 Jun: 74(6): 1265-73

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: C2C12 (ECACC 91031101).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.