C2C12
C2C12
Cell Line Name
Cell Line Description
Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.
Access Cell line profile for this product
This cell line grows quickly and differentiates quickly. It needs to be seeded at a low and density kept sub confluent below 80% maximum.
General Info
Species
Mouse
Other Collection No.
Links
Cellosaurus: CVCL_0188
Release Conditions
Characteristics
Tissue of Origin
Karyotype
Applications
Differentiation studies
Disease
Culture Conditions
Cell Type
Subculture Routine
Cells can be relatively slow growing when resuscitated from frozen taking 4-5 days to reach 50% confluence when seeded at 2x1,000 cells/cm² . Split semi-confluent cultures (50 - 70%) 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence as myotube formation may occur.
Culture Medium
DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS).
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
Nature 1977;270:725, Science 1985;230;758-766, J. Cell Biol. 1994; 127:1755-1766[published erratum appears in J Cell Biol 1995 Feb;128(4):following 713], J.Virol. 1997;71:169-178, Proc. Natl. Acad.Sci. USA 1996;93:14082-14087, J. Biol. Chem. 1996;271:1386
Bibliography
(1)J Anim Sci; 1996 Jun: 74(6): 1265-73
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: C2C12 (ECACC 91031101).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.