CD1B4
CD1B4
Cell Line Name
Cell Line Description
CD1B4 (also described in the literature as CDIB4) antibodies were isolated from centrioles and a small amount of pericentriolar material from lamb thymus cells. Monoclonal antibodies were raised by injecting denatured centriole preparations and screened using an immunofluorescence assay. The protein detected by the CD1B4 antibody is a single 96-KDa protein termed Cenexin. This is located at the base of the primary cilium, defining its association with the only mature centriole of the centriole pair. The antibody stains the mature centriole even when it is not forming a cilium and also is detected in cells which do not possess primary cilia. Immunogen: Denatured centrioles isolated from lamb thymus. Product-Antibody: Anti-cenexin. Antibody subclass: IgM.
General Info
Fusion Species
Target (Reactivity)
Also known as
Unique to ECACC
Release Conditions
Characteristics
Myeloma
Sp2-0/Ag-14
Immunogen
Denatured centrioles isolated from lamb thymus
Immunological Donor
Antibody Isotype
Morphology
Tissue of Origin
Culture Conditions
Cell Type
Subculture Routine
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO₂; 37°C
Culture Medium
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
References
References
Lange, B.M.H and Gull, K. (1995): A molecular marker for centriole maturation in the mammalian cell cycle. Journal of Cell Biology, 130: 919-927.
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: CD1B4 (ECACC 19072502)
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.