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CHO-K1.Cl-0238

CHO-K1.Cl-0238

Catalogue No.

15042911

Cell Line Name

CHO-K1.Cl-0238

Cell Line Description

Clone of CHO-K1 cells expressing Rhesus macque (Macaca mulatta) Fc?RIIIa anchored to glycosyl phosphatidylinositol. (Predicted from DQ423379 NCBI sequence)

General Info

Species

Hamster

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

Fc portion of non-human primate IgG antibody

Products

CHO cells expressing Rhesus macaque Fc?RIIIa as a glycosyl-phosphatidylinositol (GPI)-linked peptide to enable measurement of IgG binding of human, non-human, or other origin

Tissue of Origin

Ovary

Morphology

Polygonal

Karyotype

N/A

Applications

Measurement of IgG binding

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO₂/95% air; 37°C

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non- Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) with 600ug/ml hygromycin B

Growth Mode

Adherent

Additional Info

Depositor

Dr Kathryn L Armour, Cambridge Enterprise

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

Stably transfected with a pcDNA3.1/Hygro(+) based vector with a cDNA insert that had been modified to enable expression of Rhesus macaque FCγRIIIa as a GPI linked receptor

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

None specified by depositor

Bibliography

None specified by depositor

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.Cl-0238 (ECACC 15042911).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.