Catalogue No.
15042901
CHO-K1.Cl6
CHO-K1.Cl6
Cell Line Name
CHO-K1.Cl6
Cell Line Description
Clone of CHO-K1 cells expressing human FcγRIIIa of the 158V allotype anchored to glycosyl phosphatidylinositol
General Info
Species
Hamster
Unique to ECACC
Yes
Links
Cellosaurus: CVCL_DF02
Release Conditions
Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.
Characteristics
Receptors
Fc portion of human IgG antibody
Products
CHO cells expressing human FcγRIIIa of the 158V allotype as glycosyl-phosphatidylinositol (GPI)-linked peptide to enable measurement of IgG binding.
Tissue of Origin
Ovary
Morphology
Polygonal
Karyotype
N/A
Applications
Measurement of IgG binding
Disease
None Stated
Culture Conditions
Cell Type
Epithelial
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6. Seeding at 2-5 x 10,000 cells/cm² using 0.05% trypsin/EDTA; culture conditions 5% CO₂/95% air; 37°C
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non- Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) with 600ug/ml hygromycin B
Growth Mode
Adherent
Additional Info
Depositor
Dr Kathryn L Armour, Cambridge Enterprise
Country of Origin
United Kingdom
GMO Status
Genetically Modified Organism Class 1 (GMO1)
Genetic Modification
Stably transfected with a pcDNA3.1/Hygro(+) based vector with a human FCγRIIIa 158V cDNA insert that had been modified to enable expression as a GPI linked receptor
Hazard Group (ACDP)
Hazard Group (ACDP) 2
Applications
References
Armour KL, Smith CS, Clark MR. Expression of human Fc gamma RIIIa as a GPI-linked molecule on CHO cells to enable measurement of human IgG binding. J Immunol Methods. 2010 Mar 31;354 (1-2):20-33. doi: 10.1016/j.jim.2010.01.011. Epub 2010 Feb 4. PMID:20138184 [PubMed - indexed for MEDLINE
Bibliography
Armour KL, Smith CS, Turner CP, Kirton CM, Wilkes AM, Hadley AG, Ghevaert C, Williamson LM, Clark MR. Low-affinity FcγR interactions can decide the fate of novel human IgG-sensitised red blood cells and platelets. Eur J Immunol. 2014Mar; 44 (3):905-14. doi: 10.1002/eji.201343825. Epub 2014 Feb 16. PubMed Armour KL, Smith CS, Ip NC, Ellison CJ, Kirton CM, Wilkes AM, Williamson LM, Clark MR. Clearance of human IgG1-sensitised red blood cells in vivo in humans relates to the in vitro properties of antibodies from alternative cell lines. PLoS One. 2014 Oct 10;9(10):e109463. doi: 10.1371/journal.pone.0109463. eCollection 2014. PubMed PMID: 25302805; PubMed Central PMCID: PMC4193810. PMID:24285214; PubMed Central PMCID: PMC4209800. Harrison A, Liu Z, Makweche S, Maskell K, Qi H, Hale G. Methods to measure the binding of therapeutic monoclonal antibodies to the human Fc receptor FcγRIII (CD16) using real time kinetic analysis and flow cytometry. J Pharm Biomed Anal. (2012); 63:23-8. PMID:22366323 Rohatgi S, Gohil S, Kuniholm MH, Schultz H, Dufaud C, Armour KL, Badri S, Mailliard RB, Pirofski LA. Fc gamma receptor 3A polymorphism and risk for HIV-associated cryptococcal disease. MBio. 2013; 4(5):e00573-13. PMID: 23982074 PMCID: PMC3760251
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-K1.Cl6 (ECACC 15042901).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.