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UK Health Security Agency

CHO-WBL (IVGT)

CHO-WBL (IVGT)

Catalogue No.

14043001

Cell Line Name

CHO-WBL (IVGT)

Cell Line Description

The Chinese hamster ovary CHO cells were originally established as spontaneously transformed cells from an ovarian biopsy by Tijo and Puck (see references below).

Characteristics

Tissue of Origin

Ovary

Applications

Genotoxicity testing

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Pipette to break up clumps.

Culture Medium

McCoy's 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Merck Sharp and Dohme Corp.c/o Sheila M Galloway, Executive Director, Genetic Toxicology Merck Research Laboratories, W 45-316, West Point, PA 19486, USA.

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Tjio JH, Puck TT.1958 Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med.108 (2):259-68.PMID:13563760. Puck TT, Cieciura SJ, Robinson A. 1958 Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med.108 (6):945-56. PMID: 13598821.

Bibliography

Loveday KS, Anderson BE, Resnick MA, Zeiger E.1990 Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro. V: Results with 46 chemicals. Environ Mol Mutagen.16 (4):272-303. PMID: 2253606. Galloway SM, Bloom AD, Resnick M, Margolin BH, Nakamura F, Archer P, Zeiger E.1985 Development of a standard protocol for in vitro cytogenetic testing with Chinese hamster ovary cells: comparison of results for 22 compounds in two laboratories. Environ Mutagen. 7(1):1-51. PMID: 3967632. Galloway SM, Armstrong MJ, Reuben C, Colman S, Brown B, Cannon C, Bloom AD, Nakamura F, Ahmed M, Duk S, et al.1987 Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: evaluations of 108 chemicals. Environ Mol Mutagen. Suppl 10:1-175.PMID:3319609. Hu T, Miller CM, Ridder GM, Aardema MJ.1999 Characterization of p53 in Chinese hamster cell lines CHO-K1, CHO-WBL, and CHL: implications for genotoxicity testing. Mutat Res. 3; 426(1):51-62.PMID:10320750. Ivett JL, Brown BM, Rodgers C, Anderson BE, Resnick MA, Zeiger E.1989 Chromosomal aberrations and sister chromatid exchange tests in Chinese hamster ovary cells in vitro. IV. Results with 15 chemicals. Environ Mol Mutagen.; 14(3):165-87.PMID:2792092.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CHO-WBL (IVGT) (ECACC 14043001).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.