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UK Health Security Agency

CHSE-214

CHSE-214

Catalogue No.

91041114

Cell Line Name

CHSE-214

Cell Line Description

Derived from a Chinook salmon (Oncorhynchus tshawytscha) embryo, susceptible to a wide range of fish viruses and in many instances replicate high titres.

Characteristics

Tissue of Origin

Embryo

Karyotype

Not specified

Applications

Virus studies and virus titration

Disease

None Stated

Culture Conditions

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e seeding at 1-4 x 10,000 cells per cm using 0.05% trypsin or trypsin/EDTA, cells detach after 5-10 minutes at room temperature. Grow cells in dark (e.g. wrapped in foil); 5% CO₂; 21°C. Freeze cells in 5% DMSO and 95% foetal bovine serum (FBS). Fish cell lines detach easily during transit if the culture is too young. For resuscitation seed flasks at 4-5 x 10,000 cells per cm and then split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Ann NY Acad Sci 1965;126:566-586In Vitro 1984;20:671-676

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CHSE-214 (ECACC 91041114).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.