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UK Health Security Agency

Clara

Clara

Catalogue No.

07021401

Cell Line Name

Clara

Cell Line Description

A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

Characteristics

Tissue of Origin

Lung

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

Culture Medium

DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 supplements:0.25 µg/ml Endothelin-10.01 µg/ml Interferon gamma10 µg/ml Insulin5 µg/ml Transferrin7.5 µg/ml Endothelial Cell Growth Supplement (ECGS)0.025 µg/ml Epidermal Growth Factor0.36 µg/ml Hydrocortisone 0.02 µg/ml T3 For guidance on the supplements and preparation of the culture medium please refer to the product document: T7 and C22(Clara) Culture Medium.pdf

Growth Mode

Adherent

Additional Info

Depositor

Dr Daphne deMello, Department of Pathology, Phoenix Childrens Hospital, 1919 East Thomas Road, Phoenix, Arizona 85016, USA

Country of Origin

United States

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Demello et al., (2002) Generation and characterization of a conditionally immortalized lung Clara cell line from the H-2K In Vitro Cell. Dev. Biol.-Animal 38: 154-164

Bibliography

Not Available

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Clara (ECACC 07021401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.