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UK Health Security Agency

Clostridium perfringens

Clostridium perfringens

Catalogue No.

NCTC 8081

Current Name

Clostridium perfringens

Type Strain

Reference Strain

Original Strain Reference

Zeissler 2

Culture Conditions

Growth Conditions on Solid Media

Columbia blood agar, 48 hours, 37°C, AnO₂

Biological and Genomic Info

Serogroup/Serotype

Type C

Toxin Status

Alpha toxin gene positive, beta1 toxin gene positive. This isolate also contains a fragment of the enterotoxin gene and therefore has the potential to cause C. perfringens diarrhoea

Gram Stain Result

Positive

Reduction

Nitric acid: +

Enzyme Tests

Catalase: -

Cytochrome C oxidase: -

Lecithinase / alpha: +

Fermentation and Gas Production

Sucrose: +

Strain Data

(CN 2076) Human variant / H. E. Ross, NCTC in 1950 / Wellcome Collection Beckenham / S. T. Cowan / J. Zeissler, Altons, Germany / Enteritis necroticans in 1946-1948 / Note: previously listed as Type F / Oakley, C. L. (1949) Br. Med. J. i 269 / Zeissler, J. & Rassfeld-Sternberg, L. (1949) Br. Med. J. i, 267

Colony Appearance

Smooth, Shiny

Additional Info

Accession Date

03/05/1905

Depositor

ROSS H E

Isolated From

human, from case of enteritis necroticans 1946 -1948

History

ISOLATED BY ZEISSLER J-COWAN S T WELLCOME-ROSS H E NCTC CPHL PRE:FR

Previous Catalogue Name

Clostridium perfringens

Applications

Bibliography

OAKLEY C L 1949 BR MED J 1 269; ZEISSLER J & RASSFIELD-STERNBERG L 1949 BR

Extended Bibliography

Enteritis necroticans due to Clostridium welchii type F., https://www.ncbi.nlm.nih.gov/pubmed/18109335

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Clostridium perfringens (NCTC 8081)

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.