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COLO 794

COLO 794

Catalogue No.

94072237

Cell Line Name

COLO 794

Cell Line Description

Colo 794 was established from a 14 year old male patient with a malignant melanoma. The melanoma showed rapid growth and metastases in the patient and the biopsy was taken before therapy from a subcutaneous metastasis. The cell line was eradicated from mycoplasma at ECACC.COLO 794 and COLO 800 (ECACC Catalogue number 93051123) were both derived from the same 14 year old male This has been confirmed by STR profiling.

Characteristics

Tissue of Origin

Skin

Morphology

Melanocyte

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10
D5S818: 11
D7S820: 10,11
D13S317: 11,13
D16S539: 11
TH01: 6
TPOX: 11
vWA: 16,20

Karyotype

Not specified

Applications

Cancer studies

Disease

Melanoma

Culture Conditions

Cell Type

Melanocyte

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C; cells can show extensive blebs

Culture Medium

RPMI 1640+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr. G. Moore, Denver General Hospital, Colorado Oncology Foundation, Denver, Colorado, USA

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Unpublished

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 794 (ECACC 94072237).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.