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COLO 853

COLO 853

Catalogue No.

93052606

Cell Line Name

COLO 853

Cell Line Description

Derived from the right axilla lymph node of a 43 year old male with melanoma.COLO 839 (ECACC Catalogue number 93051119), COLO 858 (ECACC Catalogue number 93052613) and COLO 857 (ECACC Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma. This has been confirmed by STR profiling; COLO 853 also has an identical STR profile to the above cell lines though this cell line is not mentioned in the literature (Morse HG, Moore GE PMID: 8402545, Easty DJ et al., PMID: 7780963 and Maung K et al PMID: 10597292).

Characteristics

Products

Melanin

Tissue of Origin

Lymph node

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11
D5S818: 11
D7S820: 8,10
D13S317: 11,12
D16S539: 13
TH01: 10
TPOX: 8,11
vWA: 17,19

Karyotype

Not specified

Applications

Tumourigenicity studies

Disease

Melanoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Unpublished

Bibliography

None

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 853 (ECACC 93052606).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.