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UK Health Security Agency

COLO 858

COLO 858

Catalogue No.

93052613

Cell Line Name

COLO 858

Cell Line Description

Derived from a right axial lymph node of a 43 year old male with melanoma.COLO 839 (ECACC Catalogue number 93051119), COLO 858 (ECACC Catalogue number 93052613) and COLO 857 (ECACC Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma (see references below). This has been confirmed by STR profiling. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Characteristics

Tissue of Origin

Skin

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11
D5S818: 11
D7S820: 8,10
D13S317: 11,12
D16S539: 13
TH01: 10
TPOX: 8,11
vWA: 17,19

Karyotype

Not specified

Applications

Tumourigenicity studies

Disease

Melanoma

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 37°C; 5% CO₂.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Morse HG, Moore GE (1993) Cytogenetic homogeneity in eight independent sites in a case of malignant melanoma Cancer Genet Cytogenet. 69(2):108-12. PMID: 8402545.

Bibliography

Easty DJ, Guthrie BA, Maung K, Farr CJ, Lindberg RA, Toso RJ, Herlyn M, Bennett DC (1995) Protein B61 as a new growth factor: expression of B61 and up-regulation of its receptor epithelial cell kinase during melanoma progression. Cancer Res. 55(12):2528-32. PMID: 7780963. Maung K, Easty DJ, Hill SP, Bennett DC. (1999) Requirement for focal adhesion kinase in tumor cell adhesion. Oncogene. 18(48):6824-8. PMID: 10597292.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COLO 858 (ECACC 93052613).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.