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COR-L32

COR-L32

Catalogue No.

96020744

Cell Line Name

COR-L32

Cell Line Description

The cell line COR-L32 has been estalished from a marrow trephine from a poorly-differentiated squamous cell carcinoma. The patient has been confirmed to have primary lung cancer. Electron micrographs showed dense core granules and other features of neuroendocrine differentiation. In culture, COR-L32 grow as floating aggregates of packed cells.

Characteristics

Tissue of Origin

Bone marrow

Morphology

Floating aggregates

Karyotype

Not specified

Applications

Lung cancer study

Disease

Small Cell Carcinoma

Culture Conditions

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO₂; 37°C. This is a human small cell carcinoma which grows in large clumps. Cell banks should be frozen at high cell densities e.g. 6-9 x 1000,000 cells/ml to allow for clumping.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Aggregates in suspension

Additional Info

Depositor

Dr P Twentyman / Dr K Wright, MRC Clinical Oncology Unit, Cambridge

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Br J Cancer 1985;52:495; Human Cancer in Primary Culture 1991:199

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COR-L32 (ECACC 96020744).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.