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COR-L47

COR-L47

Catalogue No.

92031915

Cell Line Name

COR-L47

Cell Line Description

Derived from the bone marrow aspirate of a 67-year-old Caucasian male suffering from small cell lung carcinoma.

Characteristics

Products

High levels of L-dopa decarboxylase

Tissue of Origin

Lung

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,12
D5S818: 10,13
D7S820: 8,10
D13S317: 11
D16S539: 11,12
TH01: 6,8
TPOX: 8
vWA: 17

Karyotype

Not specified

Applications

Studies on lung cancer

Disease

Carcinoma

Culture Conditions

Subculture Routine

On resuscitation, cells may have low viability and can take up to 10 days to establish. However, given time, the cells will proliferate and reach ~9x105 cells/ml.  

Cells grow in very large aggregates and can be hard to count accurately. Cultures should be maintained between 3-9x105 cells/ml.

Recommended growth conditions: 5% CO2; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr P Twentyman, UKCCCR, Lincolns Inn Fields, London

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Baillie-Johnson H, Twentyman PR, Fox NE, Walls GA, Workman P, Watson JV, Johnson N, Reeve JG, Bleehen NM.1985. Establishment and characterisation of cell lines from patients with lung cancer (predominantly small cell carcinoma). Br J Cancer. 52(4):495-504 PMID: 2998422; Cancer Genet Cytogenet 1988;30:212

Bibliography

Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COR-L47 (ECACC 92031915).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.