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COV413A

COV413A

Catalogue No.

07071905

Cell Line Name

COV413A

Cell Line Description

A human ovarian epithelial-serous carcinoma cell line established from a metastasis of the sigmoid colon. This cell line was derived from the same patient as the ovarian cell line COV413B (ECACC cat no. 07071906).

Characteristics

Tissue of Origin

Ovarian metastasis

Karyotype

Not specified

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial-like/Fibroblast-like

Subculture Routine

Split confluent cultures 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA; 5% CO₂; 37°C. The cell line does not become confluent and may take up to 14 days to achieve a level suitable for subculture post resuscitation.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr C E van der Minne, S H van der Burg & P I Schrier, Department of Clinical Oncology, Leiden University Medical Centre, Albinusdreef 2 K-I-P, 2333 ZA Leiden, Netherlands.

Country of Origin

Netherlands

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

van den Berg-Bakker CA, Hagemeijer A, Franken-Postma EM, Smit VT, Kuppen PJ, van Ravenswaay Claasen HH, Cornelisse CJ, Schrier PI 1993 Establishment and characterization of 7 ovarian carcinoma cell lines and one granulosa tumor cell line: growth features and cytogenetics. Int J Cancer. 53(4):613-20 PMID: 8436435.

Bibliography

Not Available

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: COV413A (ECACC 07071905).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.