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UK Health Security Agency

CSE-119

CSE-119

Catalogue No.

95122019

Cell Line Name

CSE-119

Cell Line Description

The fish cell line CSE-119 was initiated in 1963 from pooled embryos of Coho salmon (Oncorynchus kisutch). CSE-119, also known as Coho salmon embryo-119, are susceptible to infectious pancreatic necrosis virus (IPNV) but show a lack of sensitivity to infection with viral haemorrhagic septicaemia virus (VHSV) or infectious haematopoietic necrosis virus (IHNV). This might be correlated to the natural resistance to infectious haemotopoietic necrosis in this species. For replication of salmonid viruses CSE-119 cells should be incubated at 18°C.

Characteristics

Tissue of Origin

Embryo

Karyotype

Diploid and modal no. 60

Applications

Isolation of viruses from clinical samples, virus propagation

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 15-21°C; split every 10-14 days; requires a new flask every passage. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for at least one week with 1-2 media changes before shipping.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr J L Fryer, Department of Microbiology, Oregon State University,Corvallis

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

In Vitro 1984;20:671

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CSE-119 (ECACC 95122019).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.