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UK Health Security Agency

DI TNC1

DI TNC1

Catalogue No.

98102214

Cell Line Name

DI TNC1

Cell Line Description

Established by transfecting cultures of primary astrocytes from diencephalon tissue of a 1 day old sprague-dawley rat with a DNA construct containing the oncogenic early region of SV40. Transcriptional control was affected using the human GFAP promoter (pGFA-SV-Tt) and the murine phosphoglycerate kinase promoter (pPGK-neo). Cloning was achieved using G418. The cells are phenotypically similar to type 1 astrocytes, including immunoreactivity to glial fibrillary acid protein (GFAP) and a beta-alanine inhibitable high-affinity uptake mechanism for gamma amino butyric acid (GABA). Alpha-2-macroglobulin production is similar to that found in primary astrocytes, but transferrin production is reduced. The cell line has been shown not to produce proenkephalin A, galactocerebroside or to express the 04 or A2B5 epitopes characteristic of type 2 astrocytes. Immunostaining has shown the SV40 T antigen to be present in over 90% of cells.

Characteristics

Products

Alpha-2 macroglobulin

Tissue of Origin

Brain

Karyotype

Not specified

Applications

Studies of the development and biochemistry of the central neurosystem

Disease

None Stated

Culture Conditions

Cell Type

Neuronal (astrocyte)

Subculture Routine

Split sub-confluent cultures (70-80%) at 1:2 to 1:6 using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Subculture before flasks become confluent or cells will detach in sheets reducing effectiveness of trypsin.

Culture Medium

DMEM + 4mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr C F Deschepper, IRCM, Montreal, Canada

Country of Origin

Canada

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Proc. Natl. Acad. Sci. USA 1992;89:6467

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DI TNC1 (ECACC 98102214).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.