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UK Health Security Agency

DKLP

DKLP

Catalogue No.

20082601

Cell Line Name

DKLP

Cell Line Description

DLKP was established from a poorly differentiated lung carcinoma.


Establishment of the cell line came from a lymph node biopsy of a 52y old male who smoked approximately 40 cigarettes a day for most of his adult life, and was diagnosed with poorly differentiated squamous cell lung carcinoma.


Culture designated DLKP = D=Dublin, L=Lung respectfully.

Characteristics

Tissue of Origin

Poorly differentiated lung carcinoma, established from a lymph node biopsy.

Morphology

Epithelial

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 11,12
D3S1358: 17,17
D5S818: 10,11
D7S820: 8,11
D8S1179: 13,14
D13S317: 12,12
D16S539: 10,12
D18S51: 13,13
FGA: 20,20
Penta D: 11,11
Penta E: 7,15
TH01: 9.3,9.3
TPOX: 8,11
vWA: 17,17

Karyotype

Displays chromosomal deletions and rearrangements (translocations and isochromosomes)

Applications

DLKP has been used in:
Cell line comparison studies,
Investigations into cell death in cancer cell lines,
Drug resistance studies,
Growth and attachment properties studies,
Protein expression studies
Differentiation studies

Variants derived from DLKP have been used in drug resistance studies

Disease

Carcinoma

Culture Conditions

Cell Type

Poorly differentiated squamous

Subculture Routine

Upon resuscitation count cells and seed at the higher end of the seeding density to establish culture. Feed with fresh growth medium the following day.

Seed cells between 2-4e+4 cells/cm2 and feed twice a week.

Incubate at 8% CO2 at 37°C (as medium is a mixture of DMEM/F-12 Ham's). Trypsinise using Trypsin/EDTA.

Culture Medium

DMEM:Hams F12 + 5% Foetal Bovine Serum (FBS) + 2mM L-Glutamine

Freeze in 5% DMSO; 45% FBS; 50% growth medium.

Growth Mode

Adherent

Additional Info

Depositor

Martin Clynes, National Institute for Cellular Biotechnology, Dublin City University. Originator: Geraldine Grant (1989): National Institute for Cellular Biotechnology (formerly National Cell and Tissue Culture Centre), Dublin City University.

Country of Origin

Ireland

GMO Status

Not Applicable

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Law, E., Gilvarry, U., Lynch, V., Gregory, B., Grant, G., and Clynes, M., (1992). Cytogenetic comparison of two poorly differentiated human lung squamous cell carcinoma lines. Cancer Genet. Cytogenet. 59:111-118.

Bibliography

McBride S, et al. Differentiation. (1999); 64(3):185–193. doi:10.1046/j.1432-0436.1999.6430185

O'Driscoll L, et al. Anticancer Res. 2(002);22(6A):3117-3125.

Duffy CP, Elliott CJ, O'Connor RA, et al. Eur J Cancer. 1998;34(8):1250–1259. doi: 10.1016/s0959-8049(98)00045-8

Daly P, et al. Mycopathologia. 1999;146(2):67-74. doi: 10.1023/a:1007092112873

Meleady P, Clynes M. In Vitro Cell Dev Biol Anim. 2001;37(8):536-542. doi: 10.1290/1071-2690

Meleady P, Clynes M. Cell Commun Adhes. 2001;8(1):45-59. doi: 10.3109/15419060109080706

Walsh D, et al. Differentiation. 2003;71(2):126-134. doi:

Roche S, et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877(31):3982-3990. doi:10.1016/j.jchromb.2009.10.008

Murphy L, et al. Anticancer Res. 2007;27(3A):1277-1284.

Keenan J, et al. Proteomics. 2009;9(6):1556-1566. doi:10.1002/pmic.200800633

Keenan J, Joyce H, et al (2012). Exp Cell Res 318(5):593–602.

Liang Y, et al. Int J Cancer. 2004;111(4):484-493. doi:10.1002/ijc.20230

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DKLP (ECACC 20082601).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.