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UK Health Security Agency

DMS 92

DMS 92

Catalogue No.

95062825

Cell Line Name

DMS 92

Cell Line Description

DMS 92 was obtained from a bone marrow aspirate of an untreated 39 year old male at time of diagnosis. The isolated cells were identified as small cell carcinoma of the lung (SCLC). DMS 92 cells secrete adrenocorticotropin (ACTH), bombesin (BN), oxytocin-neurophysin (OT-NP) and 17 beta-estradiol. Cells produce tumours in athymic nude mice. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Characteristics

Tissue of Origin

Lung (bone marrow metastasis)

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,13
D5S818: 11,13
D7S820: 10,11
D13S317: 12
D16S539: 12,13
TH01: 9.3
TPOX: 8,12
vWA: 16,18

Karyotype

Modal no. 44

Applications

Human lung tumour model

Disease

Small Cell Cancer

Culture Conditions

Subculture Routine

On resuscitation, a centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100 x g for 2-3 minutes to pellet cells and seed at approximately 5-7 x 105 cells/ml.

Cell line is slow to proliferate and may take up to 20 days to establish. Once established maintain cultures between 3-9 x 105 cells/ml.

Recommended growth conditions: 5% CO2, 37°C.

Culture Medium

Waymouth's MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Growth Mode

Aggregates in suspension

Additional Info

Depositor

Prof OS Pettengill and Dr G D Sorenson, Dartmouth Medical School, Lebanon, NH

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Cancer 1980;45:906; Exper Cell Biol 1980;48:279; Cancer 1981;47:1289

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: DMS 92 (ECACC 95062825).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.