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E11

E11

Catalogue No.

01110916

Cell Line Name

E11

Cell Line Description

E11 is a clone of the cell line SSN-1 and is persistantly infected with a C-type retrovirus (SnRV). It is susceptible to piscine nodavirus strains belonging to different genotypes (SJNNV, RGNNV, TPNNV and BFNNV - striped jack, red spotted grouper, tiger puffer and barfin flounder nervous necosis viruses repectively). E11 is highly permissive to nodavirus infection and production. An advantage of E11, over the parental line SSN-1, is its steady, faster growth to confluency and longer stability in monolayer cultures for 2 weeks at 25°C or 4 weeks at 20°C and its reproducible, clear CPE characterised by cytoplasmic vacuole formation followed by intensive disintegration.

Discontinued Message

Discontinued – Cell line temporarily on hold pending investigation.

General Info

Species

Fish

Release Conditions

Restricted - all organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Fish (whole)

Karyotype

Not specified

Applications

E11 will be more useful for both qualitative and quantitative analyses of piscine nodaviruses than t

Disease

None Stated

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 ie. seeding at 3 x10,000 cells/cm² using 0.05% trypsin/EDTA. No CO₂. Culture at 25°C

Culture Medium

L15 + 5% Foetal Bovine Serum (FBS)+ 2mM Glutamine

Growth Mode

Adherent

Additional Info

Depositor

Professor Toshihiro Nakai, PhD Laboratory of Fish Pathology Graduate School of Biosphere Science Hiroshima University 1-4-4 Kagamiyama, Higashi-Hiroshima Hiroshima 739-8528, Japan

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Iwamoto et al (2000), Cloning of the Fish Cell Line SSN-1 for Piscine Nodaviruses In : Diseases of Aquatic Organisms,43 p81-89

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: E11 (ECACC 01110916).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.