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Eos-HL-60

Eos-HL-60

Catalogue No.

96100920

Cell Line Name

Eos-HL-60

Cell Line Description

Eos-HL-60 is a variant of the promyelocytic cell line HL60 (ECACC catalogue no. 85011431) established by sub-cloning in methylcellulose. Although the cells are capable of reverting to the parental phenotype, most passages maintain a high degree of eosinophil differentiation.Eos-HL-60 and HL-60 have been shown to be identical genetically by STR profiling

Characteristics

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 13,14
D5S818: 12
D7S820: 11,12
D13S317: 8,11
D16S539: 11
TH01: 8
TPOX: 8,11
vWA: 16,17

Karyotype

Not specified

Applications

Study of production and function of human eosinophils, analysis of differentiation

Disease

Myelogenous Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

IDMEM + 2mM Glutamine + 0.5mM Butyric acid + 10% Foetal Bovine Serum (FBS); pH7.6; add Butyric acid to the medium just before use.

Growth Mode

Suspension

Additional Info

Depositor

Dr D W Golde, Memorial Sloan-Kettering Cancer Centre, New York

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Blood 1986;67:1433; Blood 1992;80:788

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Eos-HL-60 (ECACC 96100920).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.