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UK Health Security Agency

ES-E14TG2a

ES-E14TG2a

Catalogue No.

08021401

Cell Line Name

ES-E14TG2a

Cell Line Description

Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

Characteristics

Tissue of Origin

Embryo blastocyst

Morphology

Spheroidal, spontaneously differentiate and form embryonal structures in the absence of a feeder layer.

Applications

Studies in post-implantation structures. With conventional molecular gene modification techniques ES-E14TG2a cells can be used to reconstitute mouse embryos. When injected into blastocysts they can colonise the germ line

Disease

None Stated

Culture Conditions

Cell Type

Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells ECACC Catalogue 86032003 (irradiated or treated with mitomycin C to prevent cell division. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.

Culture Medium

DMEM + 2mM Glutamine + 1% NEAA + 0.1mM 2-Mercaptoethanol + 1000U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Foetal Bovine Serum. Renewal of medium every 2-3 days is required.

Growth Mode

Adherent

Additional Info

Depositor

Professor T Doetschman, Institute for Collaborative Research, University of Arizona, Tucson, Arizona 85721-0036. Originator: Professor M Hooper, University of Edinburgh EH4 2XU, UK.

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Smith & Hooper (1987) Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9. PMID: 3569655.

Bibliography

Kuehn et al., (1987) A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-8. PMID: 3029599.Doetschman et al., (1987) Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578. PMID: 3683574

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: ES-E14TG2a (ECACC 08021401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.