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UK Health Security Agency

Escherichia coli

Escherichia coli

Catalogue No.

NCTC 13451

Current Name

Escherichia coli

Type Strain

Reference Strain

Culture Conditions

Growth Conditions on Solid Media

Nutrient agar, 24 hours, 37°C, O₂

Biological and Genomic Info

Gram Stain Result

Negative

Strain Data

J499 = J53 E. coli Source of sequenced pEK499 plasmid (from NCTC 13441), which encodes CTX-M-15 enzyme. Plasmid pEK499 (117,536 bp) is a fusion of type FII and FIA replicons, and harbours 10 antibiotic resistance genes:blaCTX-M-15, blaOXA-1, blaTEM-1, aac6’-Ib-cr, mph(A), catB4, tet(A), and integron-borne dfrA7, aadA5 and sulI genes. GenBank accession number EU935739. Woodford et al. Antimicrob Agents Chemother. 2009 ; PMID: 19687243. Multiple antibiotic resistance plasmids

Available Formats

  • Ampoule (Bacteria)
  • Bacterial DNA - 2µg

If use of this culture results in a scientific publication, it should be cited in the publication as: Escherichia coli (NCTC 13451)

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.