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UK Health Security Agency

Escherichia coli

Escherichia coli

Catalogue No.

NCTC 14378

Current Name

Escherichia coli

Type Strain

Reference Strain

Original Strain Reference

MC1000 pDM1-mcr-2

Culture Conditions

Growth Conditions on Solid Media

Nutrient agar, 37°C, O₂, 24 hours

Biological and Genomic Info

Strain Data

This organism has been genetically modified and is GMO2 / pDM1 is a tetracycline selectable synthetic plasmid containing a p15a ori that is maintained at ~10 copies per cell. mcr-2 is cloned into the multiple cloning site of this plasmid, and is IPTG-inducible and driven by a Ptac promoter.pDM1 sequence: Genbank MN128719.The sequence of the mcr-2 ORF is available on request.

Additional Info

Accession Date

17/09/2019

Depositor

Despoina Mavridou (Imperial College London, Department of Life Sciences, Kensington, London, UK

Isolated From

GMO organism derived from Escherichia coli MC1000. London, UK.

History

Accessioned into the NCTC < Engineered by Despoina Mavridou

Applications

Bibliography

M J Casadaban, S N Cohen. J Mol Biol. 1980 Apr;138(2):179-207.L Dortet et al. Rapid detection and discrimination of chromosome- and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test. J Antimicrob Chemother. 2018 Dec 1;73(12):3359-3367.

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Escherichia coli (NCTC 14378)

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.