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UK Health Security Agency

F-36P

F-36P

Catalogue No.

99072808

Cell Line Name

F-36P

Cell Line Description

The cell line F-36P has been established from a 65 year old male patient, who was diagnosed with refractory anaemia with excess blasts (RAEB), a subtype of myelodysplastic syndrome (MDS). This non-lymphoid leukemia line was obtained from the patient's pleural fluid at time of progressed to overt leukemia. F-36P is cytokine-dependent requiring granulocyte-macrophage colony-stimulating factor (GM-CSF) or Interleukin 3 (IL-3). Cells have been shown to synthesize haemoglobin when GM-CSF or IL-3 is substituted by erythropoietin (Epo). They have been reported to be positive for leukocyte common antigen (CD45) and some multilineage markers such as CD13, CD33 and CD34, but are negative for T- and B-cell antigens and mature myelomonocytic antigens. Due to their reaction with some monoclonal antibodies recognising erythroid and platelet glycoproteins it has been suggested that F-36P has a multilineage phenotype.

Characteristics

Tissue of Origin

Bone marrow

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 9
D5S818: 13
D7S820: 9,11
D13S317: 10
D16S539: 10,12
TH01: 8
TPOX: 9,11
vWA: 14,16

Karyotype

43,Y(Xp+,-5,-7,-13,-16,-17,-19,-21,2q-,9p+,10q+,+4mar)

Applications

Study of differentiation and effect of growth signals

Disease

Myelodysplastic Syndrome

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 5ng/ml rh IL-3 + 5% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from Riken Gene Bank, Tsukuba, Japan

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Blood 1991; 78:2261, J Biol Chem 1997; 272:14334, J Biol Chem 1998; 273:22877, Mol Cell Biol 1998; 18:4282

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: F-36P (ECACC 99072808).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.