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UK Health Security Agency

F5-5.F1

F5-5.F1

Catalogue No.

99072803

Cell Line Name

F5-5.F1

Cell Line Description

Investigation of differentiation-inducible leukaemia cells is considered important in the understanding of leukaemic or neoplastic state. Several ascites lines derived from friend virus-induced erythoid leukemia show an erythroid morphology and also, unusually high haem synthesis. Most cultured lines of friend virus-induced leukemia in mice, are known to be inducible to differentiate towards erythocytes. F-5-5.F1 is susceptible to the induction by both dimethyl and butyric acid.

Characteristics

Tissue of Origin

Spleen

Karyotype

Not specified

Disease

Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100µl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37°C; 5 - 7% CO₂. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. To keep the cells in exponential growth, maintain cultures between 3-9 x100,000 cells/ml.

Culture Medium

RPMI 1640 + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Obtained from Riken Cell Bank

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Ikawa Y, Aide M, Inoue Y. Gann, 1976, 76, p767-70

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: F5-5.F1 (ECACC 99072803).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.