h1RPE7
h1RPE7
Cell Line Name
Cell Line Description
The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.
Discontinued Message
Cell line temporarily discontinued pending investigation.
General Info
Species
Human
Links
Cellosaurus: CVCL_2459
Release Conditions
Characteristics
Products
Tissue of Origin
Morphology
DNA profile (STR Profile)
Amelogenin: X
CSF1PO: 10,13
D5S818: 9,13
D7S820: 9,10
D13S317: 12
D16S539: 12
TH01: 7,9.3
TPOX: 8
vWA: 16,19
Applications
Cell line used as model for sub-retinal grafting in animals e.g. dystrophic-pigmented (RCS) rats and for in vitro analysis of retinal pigment epithelial cell biology.
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs.
Culture Medium
Hams F10 + 20% Heat-inactivated FCS + 2mM Glutamine + selection antibiotic 1µg/ml puromycin.
Growth Mode
Additional Info
Depositor
Country of Origin
GMO Status
Hazard Group (ACDP)
Applications
References
Lund et al., (2001) Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats. Proc. Natl. Acad. Sci. 98: 9942-9947 PMID: 11504951.
Bibliography
Kanuga et al., (2002) Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies. Investigative Ophthalmology & Visual Science 43: 546-555 PMID: 11818403.
Available Formats
- Frozen
- DNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: h1RPE7 (ECACC 09061602).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.