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h1RPE7

h1RPE7

Catalogue No.

09061602

Cell Line Name

h1RPE7

Cell Line Description

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

Discontinued Message

Cell line temporarily discontinued pending investigation.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Products

Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)

Tissue of Origin

Eye

Morphology

Polygonal

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10,13
D5S818: 9,13
D7S820: 9,10
D13S317: 12
D16S539: 12
TH01: 7,9.3
TPOX: 8
vWA: 16,19

Applications

Cell line used as model for sub-retinal grafting in animals e.g. dystrophic-pigmented (RCS) rats and for in vitro analysis of retinal pigment epithelial cell biology.

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 24hrs.

Culture Medium

Hams F10 + 20% Heat-inactivated FCS + 2mM Glutamine + selection antibiotic 1µg/ml puromycin.

Growth Mode

Adherent

Additional Info

Depositor

Professor John Greenwood, University College London Institute of Opthalmology, University College London, Bath Street London ECIV 9EL.

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Lund et al., (2001) Subretinal transplantation of genetically modified human cell lines attenuates loss of visual function in dystrophic rats. Proc. Natl. Acad. Sci. 98: 9942-9947 PMID: 11504951.

Bibliography

Kanuga et al., (2002) Characterization of genetically modified human retinal pigment epithelial cells developed for in vitro and transplantation studies. Investigative Ophthalmology & Visual Science 43: 546-555 PMID: 11818403.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: h1RPE7 (ECACC 09061602).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.