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H4II

H4II

Catalogue No.

89042702

Cell Line Name

H4II

Cell Line Description

From H-35 Reuber hepatoma induced by chemical carcinogenesis in an AxC rat (see Nat Cancer Inst Monogr 1964;13:229). Tyrosine aminotransferase positive. Synthesises several serum proteins including albumin, transferrin and prothrombin. (Same as H4-11-E-C3 (ECACC catalogue no. 85061112)).

Characteristics

Products

Albumin, transferrin, prothrombin

Tissue of Origin

Skin

Karyotype

52 chromosomes

Applications

Gene expression analysis

Disease

Hepatocellular Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split semi-confluent [60%-70%] cultures 1:100 i.e. seeding at 2x100 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1000,000 cells/10cm² dish.

Culture Medium

Coon's modified Ham's F12 (or Kaign's modified Ham's F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris

Country of Origin

France

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Nat Cancer Inst Monogr 1964;13:229; J Nat Cancer Inst 1961;26:891.

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: H4II (ECACC 89042702).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.