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HL60 M2

HL60 M2

Catalogue No.

88120803

Cell Line Name

HL60 M2

Cell Line Description

HL60 induced to mature into neutrophils with 1.75% DMSO after treating with SV40. Fails to express SV40 specific T Ag suggesting that HL60 M2 represents expansion of sub-clones of HL60 with inherent restricted capacity for neutrophil differentiation.

Characteristics

Tissue of Origin

Blood

Karyotype

Not specified

Applications

Differentiation studies

Disease

Myelogenous Leukaemia

Culture Conditions

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml. Do not allow the culture medium to become acidic or the cells will differentiate; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Growth Mode

Suspension

Additional Info

Depositor

Dr G Brown/Dr C M Bunce, Immunology Department The Medical School, University of Birmingham

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Exp Haematol 1983;11:828; Leukemia Res 1982;6:491

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HL60 M2 (ECACC 88120803).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.